A simple method was developed for the simultaneous determination of acebrophylline {theophylline 7-acetic acid\n(THY), ambroxol (AMB)} and montelukast sodium (MNT) in tablet dosage form by RP-HPLC. Chromatographic separation of the\ndrugs, achieved on Syncronis C-18 (250Ã?â??4.6 mm, 5Ã?¼) column at 245 nm, mobile phase comprising a mixture of\nacetonitrile:buffer in the ratio of 70:30 v/v, at a flow rate of 1 ml/min. Theophylline-7-acetic acid, ambroxol and montelukast\nsodium were eluted at the retention times of 2.04 min, 4.65 min and 6.27 min respectively. Calibration curves were linear over\nthe range of 5-250 Ã?¼g/ml, 5 Ã?¼gââ?¬â??500 Ã?¼g/ml and 5-1000 Ã?¼g/ml for theophylline-7-acetic acid, ambroxol and montelukast sodium\nwith r2 values, 0.999 and 0.998 and 0.999 respectively. The % assay of theophylline 7-acetic acid, ambroxol and montelukast\nsodium were found to be 99.54%, 100.76% and 99.2% respectively. The performance of the method was validated according to\nICH guidelines. The proposed method was successfully applied for the determination of acebrophylline and montelukast sodium\nin tablet dosage form.
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